Publication always matters in academic world. You need it for Ph.D. degree, postdoc position and, needless to say, for your job as a professor at university. There is even a saying that “Publish or Perish” among young faculty members to whom publication means everything in their career. Publication matters because it is a proof of your credibility in academic world, and thus it should be on a peer reviewed journal with reputation. (Peer review means a review of the article draft to evaluate whether it’s worth for publication by peer scientists with your identity blinded and reputation of the journal is often scored by the impact factors in SCI, scientific citation factors). Today I would like to introduce two publications about our conditioned media on skin benefits for wound healing and anti-melanogenesis (prevention of skin pigmentation).
Wound healing effect of hUCB MSC conditioned media
The first article is published by a team led by Prof. M.J. Nam of Gacheon University in the journal of Wound Repair and Regeneration (link) in 2010 about wound healing effect of conditioned media derived from hUCB MSC (human umbilical cord blood derived mesenchymal stem cell) of MEDIPOST.
If your skin is damaged by, for example, sunburn, the first thing that should happen inside the skin should be migration of fibroblast in the dermis layer to the wound site for healing. Once the fibroblast reaches the site, it produces collagen, elastin and other skin structure proteins together with anti-inflammatory and anti-oxidative molecules. The authors tested the effect of the conditioned media by treating the skin wound with a solution containing the conditioned media (test group) and with a solution without the media (control group) to confirm the migration pattern of fibroblast changed significantly in the test group compared with the control as you can see in the figure below. The number of fibroblast migrated to the wound site was 3.7 fold higher in the test group. Secondly, the fibroblast moving into the wound site should produce and secrets skin elasticity proteins such as collagen and elastin for healing. They also tested the effect of the conditioned media to find production of collagen was increased by 154% and elastin by 143%, respectively, by treatment with the media compared with control. The increase level is concentration dependent such that treatment with 5% of the conditioned media results in the increase in collagen by 1.8 fold and in elastin by 1.4 fold, respectively, compared with 2% concentration. The third step in the wound healing in skin is to maintain supplemented collagen by inhibiting collagen degrading enzymes such as MMP-1 and produce skin structure proteins like fibronectin to reconstitute damaged skin. The authors treated fibroblast with the conditioned media to see whether it shows effect on the expression of MMP-1 and fibronectin gene, respectively (expression of the corresponding gene means the protein is over-produced and activated) to find the treatment repressed MMP-1 gene expression to the level of 69.5% and activated fibronectin by 178%, respectively. One last thing is to remove activated oxygen produced in the course of wound healing process which may cause secondary damage to skin for its ultra-high reactiveness. The study found that treatment of fibroblast with conditioned media at 5% concentration increased expression level of both SOD (superoxide dismutase) and GPx (glutathione peroxidase) by 970% and 135%, respectively, both of which are major anti-oxidation enzymes in skin that remove the activated oxygen.
Skin pigmentation prevention by hUCB MSC conditioned media
Melanocyte, one of skin cells present in epidermis layer, is responsible for skin pigmentation (synthesis of melanin) and determines our skin tone color. Melanin plays a very important role in protecting our skin from the sunlight and, if distributed evenly, makes you look sexy and healthy (that’s why we do tanning at the spa). However, localization of the pigment leads to freckles or dark spot that belong to one of major skin concerns especially for Asian women. It is also noteworthy that pigmentation is somewhat related with wound healing in skin. You may have experienced freckles coming up at the end of summer when you were exposed to strong sunlight or skin remaining dark for long after being burned. Melanocyte gets activated in the course of wound healing process. There are more than hundreds cosmetic products out there with a claim of skin lightening cosmetic products or anti-melanogenesis, but none of them works to our satisfaction, unless you go to the dermatologist’s office for professional treatment such as laser. In the second paper I mentioned previously, the authors studied the anti-melanogenesis of hUCB MSC conditioned media.
They obtained melanocyte from human skin and induced pigment production by external stimulation in the presence or absence of stem cell conditioned media prepared from different sources (including umbilical cord blood) to see anti-melanogenesis effect. They confirmed the presence of stem cell conditioned media of hUCB MSC blocked the pigment synthesis and resulted in the melanin content to the level of that without external stimuli for induction (which means complete anti-melanogenesis). What’s interesting is that such anti-melanogenesis effect was found only in the presence of hUCB MSC conditioned media (as you notice the red box in the figure above). The conditioned media from other sources including adipocyte, bone marrow or skin epidermal stem cell did not show any anti-melanogenesis effect. The authors do not mention the mechanisms in detail but it seems natural to assume the hUCB MSC conditioned media is much superior to those from other sources at least in anti-melanogenesis. The melanin synthesis comes through a multi-step reactions and the first step is catalyzed by the enzyme of tyrosinase. Conditioned media of hUCB MSC was found to inhibit the activity of the enzyme in melanocyte completely and thus blocks the pigment synthesis from the very beginning. Not only the activity but also It represses the expression of the tyrosinase gene, thus blocks the synthesis of the enzyme itself. In order to simulate the in vivo environment, they also tested the anti-melanogenesis effect of the conditioned media in the artificial skin called MelanoDerm® where human melanocyte is embedded in 3 D structure mimicking human skin. The conditioned media blocked melanogenesis to the level of 45% of the naïve melanocyte without treatment of the conditioned media after induction of pigment production. They also included as an active control arbutin and resveratrol, both of which have been known to be effective in skin lightening to find the conditioned media is much superior to the active control.
MEDIPOST has conducted a clinical trial against 15 typical skin troubles including hydration, elasticity, resilience, wrinkle reduction, skin tone & brightness, soothing and dark spot & pigmentation to find NGF37 helps improvement in all these problems suffered by aging woman. The publication I introduced above is a strong science rationale for the positive outcome of the clinical trial on human.
10 AUG. 2018